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. 2019 Oct 4;23(10):496–507. doi: 10.1089/omi.2019.0122

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© Marelize Swart and Collet Dandara, 2019. Published by Mary Ann Liebert, Inc.

This Open Access article is distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.

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FIG. 1. — Comparison of mRNA expression between the three treatment conditions (efavirenz, rifampicin, and DMSO) showed no effect of efavirenz and rifampicin on mRNA expression for any of the three reference genes. Differentiated HepaRG cells were treated with efavirenz (6.4 μM), rifampicin (24.4 μM), or DMSO (0.02%) for 24 h (including three biological replicates). Expression of mRNA for three reference genes, in triplicate, were assessed by using qPCR and the fold change in mRNA expression is compared for each treatment condition and for each reference gene. (A) TBP; (B) HMBS; (C) SDHA. DMSO, dimethyl sulfoxide; HMBS, hydroxymethylbilane synthase; mRNA, messenger RNA; qPCR, quantitative polymerase chain reaction; SDHA, succinate dehydrogenase complex, subunit A, flavoprotein; TBP, TATA-binding protein.