Table 1.
Quantitative Polymerase Chain Reaction Amplification Conditions Used for Messenger RNA Expression Profiling
| Gene | Primer sequence (5′-3′) | Annealing temperature (°C) | qPCR amplification product size (bp) | References |
|---|---|---|---|---|
| CYP1A2 | F: TCGTAAACCAGTGGCAGGT; R: GGTCAGGTCGACTTTCACG | 64 | 254 | Wilkening and Bader (2003), Wilkening et al. (2003) |
| CYP2B6 | F: TTCCTACTGCTTCCGTCTATCAAA; R: GTGCAGAATCCCACAGCTCA | 62 | 67 | Antherieu et al. (2010) |
| CYP3A4 | F: CTTCATCCAATGGACTGCATAAAT; R: TCCCAAGTATAACACTCTACACAGACAA | 62 | 87 | Antherieu et al. (2010) |
| CYP3A5 | F: TGACCCAAAGTACTGGACAG; R: TGAAGAAGTCCTTGCGTGTC | 65 | 240 | Rodriguez-Antona et al. (2001) |
| UGT1A1 | F: TGACGCCTCGTTGTACATCAG; R: CCTCCCTTTGGAATGGCAC | 62 | 74 | Antherieu et al. (2010) |
| UGT2B7 | F: GGAGAATTTCATCATGCAACAGA; R: CAGAACTTTCTAGTTATGTCACCAAATATTG | 62 | 123 | Ohno and Nakajin (2009) |
| SULT1A1 | F: AACGCAAAGGATGTGGCA; R: TCCGTAGGACACTTCTCCGA | 62 | 120 | Miyano et al. (2005) |
| NR1I2 | F: CCAGGACATACACCCCTTTG; R: CTACCTGTGATGCCGAACAA | 62 | 60 | Antherieu et al. (2010) |
| NR1I3 | F: TGATCAGCTGCAAGAGGAGA; R: AGGCCTAGCAACTTCGCATA | 62 | 102 | Antherieu et al. (2010) |
| TBP | F: GAGAGTTCTGGGATTGTACCG; R: ATCCTCATGATTACCGCAGC | 62 | 143 | Ceelen et al. (2011) |
| HMBS | F: CTGTTTACCAAGGAGCTTGAAC; R: TGAAGCCAGGAGGAAGCA | 62 | 100 | Ceelen et al. (2011) |
| SDHA | F: CGGCATTCCCACCAACTACA; R: GCTGATTTTCCCACAACCTTC | 65 | 388 | Ceelen et al. (2011) |
HMBS, hydroxymethylbilane synthase; qPCR, quantitative polymerase chain reaction; SDHA, succinate dehydrogenase complex, subunit A, flavoprotein; TBP, TATA-binding protein.