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Additional file 7: A cargo particle is corralled on the membrane by the spiral ESCRT-III polymer in the flat state. The cargo binds to the dark gray receptors in the membrane but cannot bud off on its own. Switching the filament geometry from a flat to a tilted state (τ=60^∘) deforms the membrane, while switching back from the tilted to the flat state causes cargo budding where the filament is released back to the cytoplasm (see also Fig. 4a).