Table 2.
Primers for amplification of mitochondrial genome fragments
| Amplication fragments | Primer name | Sequence (5′ → 3′) | Annealing temperature (°C) |
|---|---|---|---|
| F5: COI + tRNA + COII | 5-F2637 | AGCAGGWTTTRTYCAYTGAT | 45 |
| 5-R3590 | CTCCTAAAGCWGGKAYTGTT | ||
| F7: ATP6 + COIII | 7-F4076 | ATTTTCYGTATTYGACCCYTC | 47 |
| 7-R4929 | TCTCGWGAWACATCTCGTCAT | ||
| F8: ATP6 + COIII | 8-F4518 | CGACCWGGAACWTTAGCWGT | 47 |
| 8-R5523 | TAYCCTCCTCATCARTAAAT | ||
| F19: ND1 | 19-F11982 | AAAGCAAAWCCYCCTCTTC | 47 |
| 19-R12558 | ATATTCAAATTCGTAARGG | ||
| F21: lrRNA | 21-F12834 | TTACRCCGGTTTGAACTCAG | 47 |
| 21-R13356 | WTAAAGTCTAACCTGCCCAC |