Fig. 7.

Heatmaps showing relative expression of 31 characterised P. ovis homologous allergen genes. a Stage-specific expression of P. ovis allergens: Larvae (L), Protonymph (P), Tritonymph (T), Adult Female (AF), Adult Male (AM). b Allergen expression between “fed” and “starved” P. ovis mites. Each allergen is named according to the World Health Organization and International Union of Immunological Societies (WHO/IUIS) allergen nomenclature, i.e. Pso o 1 for P. ovis Group 1 allergen. Where multiple copies of allergen genes were identified, mean expression (TPM) data was used. Relative expression shown as average TPM across three replicates/life-cycle stage/condition. Heatmaps were generated using the open source package Heatmapper [66] and Hierarchical clustering was performed using the average or unweighted pair-group method based on Pearson distance measurements