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. 2016 May 21;30(6):1150–1157. doi: 10.1002/jcla.21996

Figure 1.

Figure 1

Relative LGALS‐3 mRNA expression in the carotid plaque tissues ex vivo, according to rs2274273 (CC vs. CT+TT) and/or rs17128183 (AA vs. AG+GG), presented as 2‐ΔCt value for each tissue specimen. Quantitative real‐time PCR was performed on 39 endarterectomized human carotid plaque tissue specimens to quantify the LGALS‐3 and housekeeping Cyclophilin A relative gene expression levels. For the quantification of each PCR product, the threshold cycle (Ct) was used. The delta Ct value was calculated from the difference in the Ct of the gene of interest and that of the housekeeping gene. Data are presented as 2‐ΔCt, with mean value for both groups (CC/AA and CT+TT/AG+GG) ± SD. Relative expression levels of LGALS‐3 mRNA calculated using the REST software were significantly higher in carriers of the CT+TT or AG+GG (n = 28) comparing to carriers of the CC or AA (n = 11) (fold induction = 1.405, SE range = 0.770–2.509, P = 0.039).