Figure 2.

Applying the CRISPR-Cas12a system with paper strips to rapidly and directly visualize the result with naked eyes.

(A) Schematic of CRISPR-Cas12a system with lateral flow technology by paper strips to read out the negative or positive results. (B) Lateral flow detection of HPV16 in supernatants of SiHa cells (left) and SCC152 cells (right). (C) Lateral flow detection of HPV18 in supernatants of HeLa cells (left) and C4I cells (right). Quantification of band intensity from detection is shown below. Black bar: incubating without LbCas12a-crRNA as control. Red/orange bar: incubating with LbCas12a-crRNA that targeted HPV16 or HPV18, respectively.