Table 2.

Sequences of RPA and PCR Primers, Guide RNA, and Report Substrates

Primers for RPA and PCR
HPV16-L1_F	TTGTTGGGGTAACCAACTATTTGTTACTGTT
HPV16-L1_R	CCTCCCCATGTCTGAGGTACTCCTTAAAG
HPV18-L1_F	GCATAATCAATTATTTGTTACTGTGGTAGATACCACT
HPV18-L1_R	GCTATACTGCTTAAATTTGGTAGCATCATATTGC
Guide RNA	Sequence (5′->3′)
Cas12a crRNA-HPV16-L1	UAAUUUCUACUAAGUGUAGAUUGAAGUAGAUAUGGCAGCAC
Cas12a crRNA-HPV18-L1	UAAUUUCUACUAAGUGUAGAUACAAUAUGUGCUUCUACACA
Substrates	Sequence (5′->3′)
ssDNA-FQ reporter	/56-FAM/TTATT/3IABKFQ/
ssDNA-FB reporter	/56-FAM/TTATT/3Bio/

Abbreviations: RPA, recombinase polymerase amplification; PCR, polymerase chain reaction; and FQ, FAM-quencher.