Table 1.

Performance Characteristics of Three Different Assays

	LUMIPULSE G system	ELISA system	Agglutination assay
Principles of assays	Chemiluminescent immunoassay, CLIA (two steps)	Enzyme‐linked immunosorbent assay, ELISA (two steps)	Gelatin particle agglutination assay (one step)
Analyzer	LUMIPULSE G1200 analyzer	Tecan freedom EVOlyzer automated ELISA system	No special analyzer
Reagents	Lumipulse® G TP‐N reagent kits	ELISA Diagnostic Kits for antibody to T. pallidum	SERODIA®‐TP•PA reagents
Test time	25 min	120 min	120 min
Antibodies’ types	IgG and IgM	IgG and IgM	IgG and IgM
Coated antigen(s)	Recombination Tp15–17 and TpN47	T. pallidum antigens from genetic engineering	T. pallidum (Nichols Strain) antigen
Enzymes and substrates	ALP and AMPPD	HRP and TMB	/

ALP: alkaline phosphatase; AMPPD: 3‐[2‐spiroadamatane]‐4‐methoxy‐4‐[3‐phosphoryloxy]‐phenyl‐1,2‐dioxetane)Dioxetane ; HRP: horse radish peroxidase; TMB: tetramethylbenzidine