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Detection of CSP1 in saliva with the produced mAb against rhCSP1. CB is a Coomassie Blue‐stained SDS gel containing molecular‐size marker (lane M), purified GST‐tagged rhCSP1 as a positive control (lane 1), and human saliva (lane 2). WB is a corresponding immunoblot of CB probed with mAb‐hCSP1#4. The GST‐tagged rhCSP1 of 45 kDa was detected as shown in lanes 2 and 3. A single band at approximately 27 kDa was detected and appeared to be glycosylated CSP1.
