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. 2019 Aug 2;4:117. [Version 1] doi: 10.12688/wellcomeopenres.15368.1

Figure 1. Purification of CD1d-β 2m and SapB.

Figure 1.

A. A His-tagged construct encoding the extracellular domain of CD1d (CD1d-H 6) was co-expressed with untagged full-length β 2m in mammalian cells. B. Size exclusion chromatography (SEC) showing co-purification of CD1d-H 6 with β 2m. C. SDS-PAGE of SEC fractions. The indicated fractions containing β 2m-associated CD1d were used in subsequent experiments. D. Untagged SapB (residues 195-274 of the precursor protein PSAP) was expressed in E. coli. E. SEC of SapB after multi-step purification. F. SDS-PAGE of SEC fractions. The indicated fractions were used in subsequent experiments.