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. 2019 Aug 2;4:117. [Version 1] doi: 10.12688/wellcomeopenres.15368.1

Figure 4. Crystallisation trials of CD1d-β 2m and SapB.

Figure 4.

A. Coomassie-stained SDS-PAGE example of the purified protein sample used in crystallisation trials. B. Examples of four crystals (i)–(iv) grown in a range of conditions using different protein compositions. Scale bar represents 100 μm. C. Coomassie-stained SDS-PAGE of harvested and washed crystals shown in panel B. D. Image of the crystal that diffracted to highest resolution as described in Table 2. Scale bar represents 100 μm. Abbreviations: gCD1d-glycosylated CD1d, dgCD1d-deglycosylated CD1d, gSapB-glycosylated SapB, and ugSapB-unglycosylated SapB.