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. 2019 Oct 18;4:117. Originally published 2019 Aug 2. [Version 2] doi: 10.12688/wellcomeopenres.15368.2

Figure 2. Equilibrium binding assay of CD1d-β 2m and SapB.

Figure 2.

A. Schematic diagram illustrating the equilibrium binding assay protocol. B. Increasing concentrations of CD1d-β 2m were bound to beads and detected by Coomassie-stained SDS-PAGE. After incubation with SapB, unbound SapB was detected in supernatants by SYPRO Ruby-stained SDS-PAGE. The lipids bound to CD1d-β 2m and SapB in this experiment are the endogenous lipids they co-purify with following expression in human cells and E. coli, respectively. C. As for panel B but with CD1d-β 2m and SapB both pre-loaded with the lipid α-GalCer. D. As for panel B but with only SapB pre-loaded with the lipid α-GalCer.