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Validation of the protein extraction procedure. CAME pattern of the urine sample kept in a chamber for 0, 15, 30, 60, and 120 min after separation and the corresponding densitometric profiles (a). CAME profile of the urine sample at different loading amounts (b). Result of the SDS‐PAGE of the urine sample using four different extraction buffers (c). Result of the SDS‐PAGE of the urine sample with Laemmli sample buffer at different extraction times (d).
