Fig. 2.

Overexpression of PDE4B exacerbates OGD-triggered ER stress in HT-22 cells. (A, B) HT-22 cells were transfected with an empty plasmid (vector) or PDE4B plasmid. At 24 h after transfections, the overexpression of PDE4B was verified by Western blotting. The relative level of PDE4B/β-tubulin was measured by semiquantitative analysis of the Western blots (n = 3). (C, D, E) HT-22 cells were transfected with an empty vector or a PDE4B plasmid. At 24 h after transfections, the protein levels of GRP78, p-eIF2α, and eIF2α were determined by Western blotting. The relative levels of GRP78/β-tubulin and p-eIF2α/eIF2α were measured by semiquantitative analysis of the Western blots (n = 3). (F, G, H) HT-22 cells transfected with an empty vector or a PDE4B plasmid were subjected to 3 h of OGD, and protein levels of GRP78, p-eIF2α, and eIF2α were determined by Western blotting. The relative levels of GRP78/β-tubulin and p-eIF2α/eIF2α were measured by semiquantitative analysis of the Western blots (n = 4). Results are expressed as the mean ± SD, **P < 0.01 versus the indicated group.