Fig. 4.

FCPR03 ameliorates OGD-triggered ER stress. (A, D, F, H) HT-22 cells pretreated with FCPR03 (20 μM) for 1 h were subjected to 6 h of OGD. The protein levels of p-eIF2α, eIF2α, GRP78, p-PERK, PERK, and CHOP were determined by Western blotting. (C) HT-22 cells pretreated with FCPR03 (20 μM) for 1 h were subjected to 6 h of OGD. After fixing, cells were stained with p-eIF2α (red) and DAPI (blue). The representative images were detected by confocal microscopy. (B, E, G, I) The relative levels of p-eIF2α/eIF2α (n = 5), GRP78/β-tubulin (n = 5), p-PERK/PERK (n = 3) and CHOP/β-actin (n = 5) were determined by semiquantitative analysis of the Western blots. Results are expressed as the mean ± SD, *P < 0.05, **P < 0.01 versus the indicated group. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)