Skip to main content
. 2019 Oct 23;39(43):8457–8470. doi: 10.1523/JNEUROSCI.0016-19.2019

Figure 6.

Figure 6.

Raw has no effect upon axonal injury signaling and axonal regeneration. A, Representative examples of proximal stump of sprouting m12-Gal4-expressing motoneuron axons 20 h after nerve crush. B, Quantification of new axonal growth based on measured volume of axonal membrane within 100 μm of the injured axon tip. C, Example images showing puc-lacZ reporter expression in motoneuron nuclei that express UAS-raw-RNAi (vdrc 101255), UAS-wnd-RNAi (BL 35369) or both, together with UAS-Dcr2, via the pan-neuronal driver BG380-Gal4. D, Distribution of puc-lacZ expression levels measured in individual motoneuron nuclei coexpressing raw-RNAi; wnd-RNAi (green) compared with raw-RNAi alone (black). E, Quantification of relative puc-lacZ intensities (normalized to uninjured control) in different genotypes. Individual triangles represent mean intensities per larva measured from 30 to 35 dorsal motoneurons in segments 3–7 of the larval nerve cord. Scale bars, 20 μm. Error bars indicate SEM. ****p < 0.0001, ns, one-way ANOVA test (p > 0.05), n ≥ 11 axons in ≥ 5 animals per genotype in B, n ≥ 8 animals in 3 independent trials in E.