The protein levels of class I, IIa, IIb, and IV HDAC subtypes are up-regulated in the atherosclerotic aorta of hypercholesterolemic ApoE−/− mice. (A-C, G-I) Densitometric analysis showing the increased expression of the HDAC1, HDAC2, HDAC3, HDAC4, HDAC6, and HDAC11 proteins in the atherosclerotic aorta of ApoE−/− mice after 14 weeks on high-fat, cholesterol-rich diet. (D-F, J-L) Representative immunoblots depicting the up-regulation of HDAC subtype in the aorta of ApoE−/− (HD) as compared with ApoE−/− (ND) mice. n = 4–5, *P < 0.05, **P < 0.01, ***P < 0.001. P-values were taken in relation to ApoE−/− (ND) condition.