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. 2019 Oct 3;30:192–202. doi: 10.1016/j.molmet.2019.09.013

Figure 1.

Figure 1

NRH is a potent NAD+precursor in mammalian cells. (A) Chemical structure of NRH. (B) AML12 cells were treated with different doses of NR or NRH and 1 h later acidic extracts were obtained to measure NAD+ levels. * indicates statistical difference at p < 0.05 vs the respective 0 mM group. (C) AML12 cells were treated with 0.5 mM NR or NRH for the times indicated. Acidic extracts were obtained to measure NAD+ levels. * indicates statistical difference at p < 0.05 vs the respective t=0 group. (D) C2C12 myotubes, INS1E cells, or 3T3 fibroblasts were treated with either PBS (as a vehicle) or NRH (0.5 mM) for 1 h prior to the evaluation of intracellular NAD+ levels. * indicates statistical difference at p < 0.05 vs the vehicle-treated group. All the values in the figure are expressed as mean+/-SEM of n = 3–5 independent experiments.