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. 2019 Oct 3;30:192–202. doi: 10.1016/j.molmet.2019.09.013

Figure 3.

Figure 3

NRH is orally bioavailable and does not degrade in plasma. (A) Mouse plasma was isolated and then spiked with either NR (0.5 mM) or NRH (0.5 mM). Samples were then snap frozen immediately after spiking with the compound or after 2 h of incubation at 37 °C. NAM, NR, and NRH were measured by LC-MS. The results are presented as the peak signal corrected by internal standard (IS). * indicates statistical difference vs the respective t = 0 group at p < 0.05. (B) Eight-week-old C57Bl/6NTac mice were intraperitoneally injected with either saline (as vehicle), NR or NRH at either 250 or 500 mg/kg. One hour later, tissue samples were collected and immediately frozen. Acidic extracts were obtained from the liver, muscle, and kidney to measure NAD+ levels. (C) Evaluation of NAM, NR, and NRH levels in the mice plasma 1 h after the intraperitoneal injection of saline (as a vehicle) or 250 mg/kg of NR or NRH. (D–E) Eight-week-old C57Bl/6NTac mice were orally gavaged with either saline (as a vehicle), NR (500 mg/kg), or NRH (500 mg/kg). After 1 h, hepatic NAD+ levels (D) and circulating levels of NRH and NAM (E) were evaluated as in (C). All the results are expressed as mean+/-SEM of 3 independent experiments (A) or n = 5 mice per group (B–E). In panels B–E, * indicates statistical difference at p < 0.05 vs the saline-treated mice. # indicates statistical difference at p < 0.05 vs the NR-treated mice at the respective dose.