Figure 4.

NRH protects against cisplatin-induced acute kidney injury. Eight-week-old C57Bl/6NTac mice were intraperitoneally injected with either saline (control group) or cisplatin (20 mg/kg). Simultaneously, the mice were intraperitoneally injected or not injected with PBS (vehicle) or NRH (250 mg/kg). PBS or NRH were then injected at 24, 48, and 72 h after the initiation of the experiment, immediately after collecting urine. The mice were sacrificed 4 h after the last injection, and urine, blood, and kidneys were collected. (A) NAD⁺ metabolites levels in the kidney were measured by mass spectrometry. The results are expressed as the peak signal corrected by internal standard (IS) and protein. (B) Total kidney protein extracts were used to measure poly-ADPribosylation levels and PARP1. Tubulin was used as a loading control. Quantifications for these blots can be found in Supplementary Fig. 3B. (C) Blood urea nitrogen levels in the mice plasma. (D) Urea levels in urine 24 h after the initiation of the experiment. (E) H&E staining of the kidney. Bar length: 100 μM. (F) Quantification of kidney casts from histology images. (G) Total mRNA was extracted from the kidney to evaluate the markers indicated by RT-qPCR. All the values are expressed as mean+/-SEM of n = 4 mice per group. * indicates statistical difference at p < 0.05 between the respective NRH and vehicle-injected mice. ^# indicates statistical difference at p < 0.05 between the respective cisplatin and control groups.