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. 2019 Sep 25;6:2329–2347. doi: 10.1016/j.mex.2019.09.029

Table 2.

Fresh frozen Rehydration.

1. Set up 6 plastic Coplin staining jars with 100% ethanol, 90% ethanol, 70% ethanol, 50% ethanol, 30% ethanol, LC-MS water
2. Immerse the slide in 100% ethanol for 2 min, 90% ethanol for 2 min, 70% ethanol for 2 min, 50% ethanol for 2 min, 30% ethanol for 2 min, wash in LC-MS water and keep slide submerged until transfer for digestion.