Figure 3.

Deregulated lncRNAs in Extracellular Vesicles and Cardiomyocytes during Hypoxia/Reoxygenation

(A) Heatmap of lncRNA mouse array in cardiomyocytes, sEVs, and lEVs exposed to hypoxic conditions for 24 h following 4 h of reoxygenation or normoxic conditions for 28 h. n = 3 independent experiments. (B and C) Different lncRNAs are (B) upregulated and (C) downregulated during hypoxia/reoxygenation in lEVs, sEVs, and cells, and only 12 (green, downregulated) or 28 (red, upregulated) are shared by all. (D–F) Scheme of the selection strategy (D) to identify lncRNA candidates in lEVs and sEVs leading to 2 candidates: Ensembl: ENSMUST00000122745 in sEVs (E) and Neat1 in lEVs (F). n = 3–6 independent experiments. Cells = cardiomyocytes; ctrl = normoxia; hyp = hypoxia/reoxygenation.