Figure 1.

Experimental Design

(A) Timeline (in months) of injection procedures, sample collection, and in-life assays. Baseline samples were collected on day “0,” immediately prior to the first subretinal injection. Similarly, samples were collected again immediately prior to the second subretinal injection on day 60. Blood samples were then collected every 2 weeks until termination of the study. Bl, phlebotomy; ACP, anterior chamber paracentesis; OCT, optical coherence tomography; Vit, vitreous biopsy. (B) Cartoons depicting the location and extent of the blebs created after the bilateral subretinal injections in each of the three animals for the first (violet) and second (turquoise) administrations for each eye. Regions of overlap of the two injections appear in blue. Right eye is the leftward-most image of each pair of cartoons per animal (i.e., cartoons are depicted as if one were looking into the animal’s eyes).