Table 1.
Results of Computer and Experimental BT2‐RFLP Analysis With FatI, Alw21I, HpyCH4V, and MwoI for Differentiation of Dermatophyte Strains Tested in This Study
| Species | BT2 GenBank accession number used for in silico analysis | Total number of tested strains in this study | Size of the BT2 amplicon (bp) | BT2 profile after digestion with FatI (bp) | BT2 profile after digestion with Alw21I (bp) | BT2 profile after digestion with HpyCH4V (bp) | Profile after digestion with MwoI | Species identification by BT2‐RFLP/ sequencing |
|---|---|---|---|---|---|---|---|---|
| T. interdigitale | JF731050–JF731054 | 55 | 790 | 2, 202, 246, 340 | 152, 217, 421 | NA | NA | T. interdigitale |
| T. tonsurans | JF731070–JF731074 | 9 | 790 | 2, 202, 246, 340 | 217, 573 | NA | NA | T. tonsurans |
| T. rubrum | JF731063–JF731067 | 28 | 796 | 2, 63, 125, 246, 360 | NA | NA | 9, 97, 180, 510 | T. rubrum |
| T. violaceum | JF731088–JF731089 | 5 | 796 | 2, 63, 125, 246, 360 | NA | NA | 9, 98, 689 | T. violaceum |
| M. gypseum | JF731096–JF731098 | 5 | 798 | 2, 124, 672 | NA | NA | NA | M. gypseum |
| M. canis | JF731101–JF731103 | 10 | 770 | 2, 68, 111, 117, 226, 246 | NA | NA | NA | M. canis |
| T. schoenleinii | JF731081–JF731082 | 2 | 798 | 2, 246, 550 | NA | 83, 118, 125, 219, 253 | NA | T. schoenleinii |
| E. floccosum | JF731122–JF731123 | 24 | 789 | 2, 246, 541 | NA | 81, 219, 489 | NA | E. floccosum |
| T. verrucosum | Undeposited data | 2 | 798 | 124, 250, 424 | NA | NA | NA | T. verrucosum |
NA, not applicable.