Multiplex‐PCR amplification pattern for DEC positive controls. (A) (1) 100 pb DNA ladder (Fermentas), (2) STEC (stx1 stx2 eae), (3) EPEC (eae, bfpA). (B) (1) 100 pb DNA ladder (Fermentas), (2) ETEC (lt, st), (3) EIEC (ial). (C) (1) 100 pb DNA ladder (Fermentas), (2) EIEC (ipaH), (3) EAEC (CVD432), (4) DAEC (daaE). A band of approximately 1,000 pb seen in all gels correspond to the internal amplification control 16S rRNA gene. (A) and (B) correspond to the multiplex‐PCR system 1 target genes, and (C) to system 2 target genes.