Table 1.
Primers Used for Detection of Diarrheagenic E. coli and Internal Amplification Control
| DEC | Primer | Sequence | Amplicon | |
|---|---|---|---|---|
| group | (concentration μM) | (5′–3′) | size (pb) | Reference |
| STEC | stx1 | F‐CTGGATTTAATGTCGCATAGTG | 150 | 15, 16 |
| (0.4) | R‐AGAACGCCCACTGAGATCATC | |||
| stx2 | F‐GGCACTGTCTGAAACTGCTCC | 255 | 15 | |
| (0.48) | R‐TCGCCAGTTATCTGACATTCTG | |||
| EPEC | bfpA | F‐AATGGTGCTTGCGCTTGCTGC | 324 | 17 |
| (0.48) | R‐GCCGCTTTATCCAACCTGGTA | |||
| eae | F‐GACCCGGCACAAGCATAAGC | 384 | 15 | |
| (0.48) | R‐CCACCTGCAGCAACAAGAGG | |||
| ETEC | lt | F‐GGCGACAGATTATACCGTGC | 450 | 16 |
| (0.48) | R‐CGGTCTCTATATTCCCTGTT | |||
| st | F‐ATTTTTMTTTCTGTATTRTCTT | 190 | 17 | |
| (0.48) | R‐CACCCGGTACARGCAGGATT | |||
| EIEC | ial | F‐GGTATGATGATGATGAGTCCA | 650 | 16 |
| (0.6) | R‐GGAGGCCAACAATTATTTCC | |||
| ipaH | F‐GTTCCTTGACCGCCTTTCCGATACCGTC | 600 | 18 | |
| (0.48) | R‐GCCGGTCAGCCACCCTCTGAGAGTAC | |||
| EAEC | CVD432 | F‐CTGGCGAAAGACTGTATCAT | 630 | 18 |
| (0.48) | R‐CAATGTATAGAAATCCGCTGTT | |||
| DAEC | daaE | F‐GAACGTTGGTTAATGTGGGGTAA | 542 | 19 |
| (0.48) | R‐TATTCACCGGTCGGTTATCAGT | |||
| Internal | 16S | F‐CCAGCAGCCGCGGTAATACG | 996 | 20 |
| Control | (0.48) | R‐ATCGGYTACCTTGTTACGACTTC |