Table 2.

Validation of rRT‐PCR Array Sensitivity

		Ct value
Assay	Number of RNA (copies/reaction tube)	RT‐PCR array	Individual RT‐PCR	Standard PCR
HA	100,000	21.79	21.19	＋
	10,000	25.54	26.07	＋
	1,000	30.78	29.77	＋
	100	34.16	33.84	−
	10	37.67	37.23	−
	1	−	−	−
H1N1	100,000	19.50	19.77	＋
	10,000	22.50	22.61	＋
	1,000	25.13	26.48	＋
	100	28.34	28.30	−
	10	35.37	34.97	−
	1	−	−	−
H5N1	100,000	21.77	21.46	＋
	10,000	25.33	25.43	＋
	1,000	29.13	29.38	＋
	100	33.54	32.73	＋
	10	37.08	36.36	−
	1	−	−	−
H9N2	100,000	19.97	20.14	＋
	10,000	23.28	23.49	＋
	1,000	27.33	27.41	＋
	100	31.24	31.38	−
	10	36.04	35.18	−
	1	−	−	−

Positive (+) and negative detections (−) are indicated