Table 4.
Intra‐ and Interassay Reproducibility of the rRT‐PCR and AIV RT‐PCR Arrays
Intraassay | Interassay | ||||||
---|---|---|---|---|---|---|---|
Assay | Number of RNA copies | Mean Ct | SD | CV (%) | Mean Ct | SD | CV (%) |
HA | 106 | 18.95 | 0.325 | 1.71 | 19.31 | 0.629 | 3.26 |
104 | 27.78 | 0.241 | 0.87 | 27.62 | 0.617 | 2.23 | |
102 | 34.88 | 0.096 | 0.28 | 34.86 | 0.181 | 0.51 | |
H1N1 | 106 | 15.86 | 0.341 | 2.15 | 15.98 | 0.235 | 1.47 |
104 | 22.50 | 0.401 | 1.78 | 23.06 | 0.742 | 3.21 | |
102 | 28.30 | 0.453 | 1.6 | 29.13 | 0.979 | 3.36 | |
H5N1 | 106 | 18.08 | 0.118 | 0.65 | 18.81 | 0.631 | 3.35 |
104 | 25.33 | 0.278 | 1.1 | 25.62 | 0.625 | 2.44 | |
102 | 32.20 | 0.780 | 2.42 | 33.22 | 0.792 | 2.38 | |
H9N2 | 106 | 21.61 | 0.306 | 1.42 | 21.226 | 0.908 | 4.28 |
104 | 28.77 | 0.158 | 0.55 | 28.559 | 0.580 | 2.03 | |
102 | 35.77 | 0.173 | 0.48 | 35.556 | 0.300 | 0.84 |