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. 2013 Nov 11;27(6):450–460. doi: 10.1002/jcla.21627

Table 4.

Intra‐ and Interassay Reproducibility of the rRT‐PCR and AIV RT‐PCR Arrays

Intraassay Interassay
Assay Number of RNA copies Mean Ct SD CV (%) Mean Ct SD CV (%)
HA 106 18.95 0.325 1.71 19.31 0.629 3.26
104 27.78 0.241 0.87 27.62 0.617 2.23
102 34.88 0.096 0.28 34.86 0.181 0.51
H1N1 106 15.86 0.341 2.15 15.98 0.235 1.47
104 22.50 0.401 1.78 23.06 0.742 3.21
102 28.30 0.453 1.6 29.13 0.979 3.36
H5N1 106 18.08 0.118 0.65 18.81 0.631 3.35
104 25.33 0.278 1.1 25.62 0.625 2.44
102 32.20 0.780 2.42 33.22 0.792 2.38
H9N2 106 21.61 0.306 1.42 21.226 0.908 4.28
104 28.77 0.158 0.55 28.559 0.580 2.03
102 35.77 0.173 0.48 35.556 0.300 0.84