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HPLC Parameters
| Time (min) | Events | Parameter (%) |
|---|---|---|
| 0.00 | Pump B | 80 |
| 4.00 | Pump B | 100 |
| 5.00 | Pump B | 100 |
| 6.00 | Pump B | 80 |
| 6.10 | Stop |
Column temperature was 45°C and the flow rate was 1 mL/min. Gradient was made from 100% water containing 0.1% formic acid (A) and 100% methanol containing 0.1% formic acid (B).
