Table 1.

Analytical Characteristics and Calibration Procedures of Dimension Vista® Methods

Analyte	Mnemonic	Calibrator	Calibration frequency	Calibration type	Sample (μl)a	Reagent 1	Reagent 2	Reagent 3	Reaction time min	Principle of methods/technology	Wavelength (nm)
α1‐acid glycoprotein	A1AG	PROT1 CAL	45 days	non linear 6 points	7.7a	127.6	7	15.3	6	Specific antibodies/Nephelometry	840
α 1‐antitrypsin	A1AT	PROT1 CAL	45 days	non linear 6 points	13.7a	109.6	27.4		6	Specific antibodies/Nephelometry	840
Albumin	ALB	CHEM 4 CAL	90 days	linear 2 points	1.67	41.7			2.2	Bromocresol purple/polychromatism end point	540, 600, 700
Alkaline Phosphatase	ALP	ALP CAL	90 days	linear 2 points	3	6	19.29	19.29	5.6	AMP/Calibration/37°C/bichromatic kinetic	405, 510
Alanine Aminotransferase	ALT	ENZ 2 CAL	90 days	linear 2 points	14.58	12.5	33.33		5.3	L‐Alanine/α‐KG/NADH in NAD/Calibration/37°C/bichromatic kinetic	340, 700
Amylase	AMY	ENZ 1 CAL	90 days	linear 2 points	5.25	82.1			3.4	CNPG3/ Calibration/37°C / bichromatic kinetic	405, 577
Aspartate Aminotransferase	AST	ENZ 2 CAL	90 days	linear 2 points	13.33	33.33	21.67		5.3	L‐Aspartate/α KG/NADH in NAD/Calibration/37°C/bichromatic kinetic	340, 700
Beta HCG	BHCG	BHCG CAL	30 days	non linear 6 points	2	25	25	100	10	LOCI/Chemiluminescence (flash)	680, 612
Urea	UREA	CHEM 1 CAL	30 days	linear 2 points	1.2	36			2.6	Urease and GLDH/bichromatic kinetic	340, 383
Calcium	CA	CHEM 1 CAL	90 days	linear 2 points	1.67	48.33	11		1.2	Cresolphthalein Complexone/bichromatic end point	577, 540
Cholesterol	CHOL	CHEM 1 CAL	90 days	linear 2 points	1.25	36.5	10.8		5.6	Cholesterol esterase (Stadtman adapté/Liedtke) bichromatic end point	540, 452,700
Creatine Kinase	CK	CK CAL	90 days	linear 2 points	5.77	39.15			4.9	CP/ADP/NADP in NADPH/Calibration/37°C/bichromatic kinetic	340, 405
Creatinine	CREA	CHEM 1 CAL	90 days	linear 2 points	8	29.6	7.2		1.2	Picrate in alkaline buffer(Jaffé)/bichromatic kinetic	510, 600
CRP	CRP	PROT2 CAL	45 days	non linear 7 points	1.37a	118	27.3		5.8	Specific antibodies/Nephelometry	840
Cardiac Troponin I	cTnI	cTnI CAL	30 days	non linear 6 points	20	20	20	13	10	LOCI/Chemiluminescence (flash)	680, 612
Direct Bilirubin	DBIL	TDBIL CAL	90 days	linear 2 points	5	12.5	25		4.9	Diazo‐Caffeine/Benzoate (Jendrassik‐Grof) w/blank/bichromatic end point	540, 700
Ferritin	FERR	LOCI 4 CAL	30 days	non linear 5 points	2	20	20	100	10	LOCI/Chemiluminescence (flash)	680, 612
γ Glutamyl Transferase	GGT	ENZ 1 CAL	90 days	linear 2 points	11.43	26.79			1.9	GCNA/Calibration/37°C/bichromatic kinetic	405, 600
Glucose	GLU	CHEM 1 CAL	90 days	linear 2 points	1.2	22.4			2	Hexokinase, UV/bichromatic end point	340, 383
Haptoglobin	HAPT	PROT1 CAL	45 days	non linear 6 points	7.1a	114	7	28.6	6	Specific antibodies/Nephelometry	840
hsCRP	hsCRP	PROT2 CAL	45 days	non linear 7 points	1.37	118	27.3		5.8	Specific antibodies/Nephelometry	840
Lactic Acid	LA	CHEM 1 CAL	90 days	linear 2 points	1.8	9	33.8	9	8.9	Lactate in Pyruvate/NAD in NADH/Calibration/37°C/bichromatic end point	340, 383
Lactate Dehydrogenase	LDH	ENZ 1 CAL	90 days	linear 2 points	6	21.43	38.57		2	L‐Lactate/NAD in NADH/Calibration/37°C/bichromatic kinetic	340, 383
Lipase	LIP	ENZ 1 CAL	45 days	linear 2 points	1.9	48	48		3.1	Methyl resorufin ester substrate/Calibration/37°C/bichromatic kinetic	577, 700
Microalbuminuria	MALB	PROT3 CAL	45 days	non linear 7 points	10	117.5	20		19	Specific antibodies/Nephelometry	840
N‐terminal pro‐BNP	PBNP	PBNP CAL	30 days	non linear 6 points	8	20	20	15	10	LOCI/Chemiluminescence (flash)	680, 612
Phosphorus	PHOS	CHEM 2 CAL	90 days	linear 2 points	1.3	21.3	8.5	8.5	2.9	Phosphomolybdate UV/bichromatic end point	340, 700
Triglycerides	TG	CHEM 2 CAL	90 days	linear 2 points	1.6	55			5.6	Lipoprotein Lipase‐ GK‐ GPO‐ POD bichromatic end point	510, 700
Total Bilirubin	TBIL	TDBIL CAL	90 days	linear 2 points	5	125	23.5		7.5	Diazo‐(J‐G) w/blank/bichromatic end point	540, 700
Total CO2	CO2	CHEM 3 CAL	90 days	linear 2 points	1.9	38			1.9	Enzymatic/bichromatic kinetic	405, 700
Total proteins	TP	CHEM 4 CAL	90 days	linear 2 points	6.2	34.7	34.7		3.8	Biuret/bichromatic end point	540, 700
Transferrin	TRF	PROT1 CAL	45 days	non linear 5 points	7.1a	114.3	7	28.6	6	Specific antibodies/Nephelometry	840
Urine/CSF proteins	UCFP	UCFP CAL	60 days	non linear 5 points	3.7	128.68			2	Pyrogallol red/bichromatic end point	600, 700
Uric Acid	URCA	CHEM 1 CAL	90 days	linear 2 points	6.6	50.8	10		4.9	Uricase/bichromatic end point	293, 700
Na, K, Cl	Na, K, Cl	STD A – B	4 hr		25				0.35	Indirect Potentiometry

^a1/20 prediluted sample. The indicated volume is in the reaction cup.