Table 1.
Analyte | Mnemonic | Calibrator | Calibration frequency | Calibration type | Sample (μl)a | Reagent 1 | Reagent 2 | Reagent 3 | Reaction time min | Principle of methods/technology | Wavelength (nm) |
---|---|---|---|---|---|---|---|---|---|---|---|
α1‐acid glycoprotein | A1AG | PROT1 CAL | 45 days | non linear 6 points | 7.7a | 127.6 | 7 | 15.3 | 6 | Specific antibodies/Nephelometry | 840 |
α 1‐antitrypsin | A1AT | PROT1 CAL | 45 days | non linear 6 points | 13.7a | 109.6 | 27.4 | 6 | Specific antibodies/Nephelometry | 840 | |
Albumin | ALB | CHEM 4 CAL | 90 days | linear 2 points | 1.67 | 41.7 | 2.2 | Bromocresol purple/polychromatism end point | 540, 600, 700 | ||
Alkaline Phosphatase | ALP | ALP CAL | 90 days | linear 2 points | 3 | 6 | 19.29 | 19.29 | 5.6 | AMP/Calibration/37°C/bichromatic kinetic | 405, 510 |
Alanine Aminotransferase | ALT | ENZ 2 CAL | 90 days | linear 2 points | 14.58 | 12.5 | 33.33 | 5.3 | L‐Alanine/α‐KG/NADH in NAD/Calibration/37°C/bichromatic kinetic | 340, 700 | |
Amylase | AMY | ENZ 1 CAL | 90 days | linear 2 points | 5.25 | 82.1 | 3.4 | CNPG3/ Calibration/37°C / bichromatic kinetic | 405, 577 | ||
Aspartate Aminotransferase | AST | ENZ 2 CAL | 90 days | linear 2 points | 13.33 | 33.33 | 21.67 | 5.3 | L‐Aspartate/α KG/NADH in NAD/Calibration/37°C/bichromatic kinetic | 340, 700 | |
Beta HCG | BHCG | BHCG CAL | 30 days | non linear 6 points | 2 | 25 | 25 | 100 | 10 | LOCI/Chemiluminescence (flash) | 680, 612 |
Urea | UREA | CHEM 1 CAL | 30 days | linear 2 points | 1.2 | 36 | 2.6 | Urease and GLDH/bichromatic kinetic | 340, 383 | ||
Calcium | CA | CHEM 1 CAL | 90 days | linear 2 points | 1.67 | 48.33 | 11 | 1.2 | Cresolphthalein Complexone/bichromatic end point | 577, 540 | |
Cholesterol | CHOL | CHEM 1 CAL | 90 days | linear 2 points | 1.25 | 36.5 | 10.8 | 5.6 | Cholesterol esterase (Stadtman adapté/Liedtke) bichromatic end point | 540, 452,700 | |
Creatine Kinase | CK | CK CAL | 90 days | linear 2 points | 5.77 | 39.15 | 4.9 | CP/ADP/NADP in NADPH/Calibration/37°C/bichromatic kinetic | 340, 405 | ||
Creatinine | CREA | CHEM 1 CAL | 90 days | linear 2 points | 8 | 29.6 | 7.2 | 1.2 | Picrate in alkaline buffer(Jaffé)/bichromatic kinetic | 510, 600 | |
CRP | CRP | PROT2 CAL | 45 days | non linear 7 points | 1.37a | 118 | 27.3 | 5.8 | Specific antibodies/Nephelometry | 840 | |
Cardiac Troponin I | cTnI | cTnI CAL | 30 days | non linear 6 points | 20 | 20 | 20 | 13 | 10 | LOCI/Chemiluminescence (flash) | 680, 612 |
Direct Bilirubin | DBIL | TDBIL CAL | 90 days | linear 2 points | 5 | 12.5 | 25 | 4.9 | Diazo‐Caffeine/Benzoate (Jendrassik‐Grof) w/blank/bichromatic end point | 540, 700 | |
Ferritin | FERR | LOCI 4 CAL | 30 days | non linear 5 points | 2 | 20 | 20 | 100 | 10 | LOCI/Chemiluminescence (flash) | 680, 612 |
γ Glutamyl Transferase | GGT | ENZ 1 CAL | 90 days | linear 2 points | 11.43 | 26.79 | 1.9 | GCNA/Calibration/37°C/bichromatic kinetic | 405, 600 | ||
Glucose | GLU | CHEM 1 CAL | 90 days | linear 2 points | 1.2 | 22.4 | 2 | Hexokinase, UV/bichromatic end point | 340, 383 | ||
Haptoglobin | HAPT | PROT1 CAL | 45 days | non linear 6 points | 7.1a | 114 | 7 | 28.6 | 6 | Specific antibodies/Nephelometry | 840 |
hsCRP | hsCRP | PROT2 CAL | 45 days | non linear 7 points | 1.37 | 118 | 27.3 | 5.8 | Specific antibodies/Nephelometry | 840 | |
Lactic Acid | LA | CHEM 1 CAL | 90 days | linear 2 points | 1.8 | 9 | 33.8 | 9 | 8.9 | Lactate in Pyruvate/NAD in NADH/Calibration/37°C/bichromatic end point | 340, 383 |
Lactate Dehydrogenase | LDH | ENZ 1 CAL | 90 days | linear 2 points | 6 | 21.43 | 38.57 | 2 | L‐Lactate/NAD in NADH/Calibration/37°C/bichromatic kinetic | 340, 383 | |
Lipase | LIP | ENZ 1 CAL | 45 days | linear 2 points | 1.9 | 48 | 48 | 3.1 | Methyl resorufin ester substrate/Calibration/37°C/bichromatic kinetic | 577, 700 | |
Microalbuminuria | MALB | PROT3 CAL | 45 days | non linear 7 points | 10 | 117.5 | 20 | 19 | Specific antibodies/Nephelometry | 840 | |
N‐terminal pro‐BNP | PBNP | PBNP CAL | 30 days | non linear 6 points | 8 | 20 | 20 | 15 | 10 | LOCI/Chemiluminescence (flash) | 680, 612 |
Phosphorus | PHOS | CHEM 2 CAL | 90 days | linear 2 points | 1.3 | 21.3 | 8.5 | 8.5 | 2.9 | Phosphomolybdate UV/bichromatic end point | 340, 700 |
Triglycerides | TG | CHEM 2 CAL | 90 days | linear 2 points | 1.6 | 55 | 5.6 | Lipoprotein Lipase‐ GK‐ GPO‐ POD bichromatic end point | 510, 700 | ||
Total Bilirubin | TBIL | TDBIL CAL | 90 days | linear 2 points | 5 | 125 | 23.5 | 7.5 | Diazo‐(J‐G) w/blank/bichromatic end point | 540, 700 | |
Total CO2 | CO2 | CHEM 3 CAL | 90 days | linear 2 points | 1.9 | 38 | 1.9 | Enzymatic/bichromatic kinetic | 405, 700 | ||
Total proteins | TP | CHEM 4 CAL | 90 days | linear 2 points | 6.2 | 34.7 | 34.7 | 3.8 | Biuret/bichromatic end point | 540, 700 | |
Transferrin | TRF | PROT1 CAL | 45 days | non linear 5 points | 7.1a | 114.3 | 7 | 28.6 | 6 | Specific antibodies/Nephelometry | 840 |
Urine/CSF proteins | UCFP | UCFP CAL | 60 days | non linear 5 points | 3.7 | 128.68 | 2 | Pyrogallol red/bichromatic end point | 600, 700 | ||
Uric Acid | URCA | CHEM 1 CAL | 90 days | linear 2 points | 6.6 | 50.8 | 10 | 4.9 | Uricase/bichromatic end point | 293, 700 | |
Na, K, Cl | Na, K, Cl | STD A – B | 4 hr | 25 | 0.35 | Indirect Potentiometry |
1/20 prediluted sample. The indicated volume is in the reaction cup.