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. 2019 Oct 11;25(8):1155–1163. doi: 10.1089/mdr.2018.0414

FIG. 3.

FIG. 3.

SDS-PAGE analysis of OMPs of Klebsiella pneumoniae isolates. M: Marker; C: ATCC 13883; 1-7662: K. pneumoniae isolates. The SDS-PAGE analysis of OMPs extracted from K. pneumoniae isolates in nutrient broth (−) or nutrient broth with 20% sorbitol (+) overnight. M: Protein molecular weight marker; ATCC13883: control; 1-7662: K. pneumoniae isolates. The 12% SDS-PAGE was performed with 20 μg of isolated outer membrane proteins with 6 M urea. The band identified as OmpK36 by trypsin digestion following MALDI-TOF/MS-MS analysis is marked by a white arrow, and the position of OmpK35 and OmpA band is also indicated (black arrow and arrowhead, respectively). OMP, outer membrane protein; SDS-PAGE, sodium dodecyl sulfate-polyacrylamide gel electrophoresis.