Figure 5.
Representative confocal microscopy images of 43 h-old S. mutans and C. albicans dual- and single-species biofilm. The green color represents the microbial content in each biofilm (labeled with SYTO9), while the red color represents ESP in the extracellular matrix produced by S. mutans Gtfs (labeled with Alexa Fluor 647). The images in the first column show the overlay of both components, while the second and third images illustrate each component individually. The white arrow shows hyphae of C. albicans surrounded by exopolysaccharides produced by S. mutans. Sm: S. mutans and Ca: C. albicans.