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. 2019 Jul 30;3(9):e10199. doi: 10.1002/jbm4.10199

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© 2019 The Authors. JBMR Plus published by Wiley Periodicals, Inc. on behalf of American Society for Bone and Mineral Research.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Quantification of Prrx1 + and PCNA + periosteal cells from immunohistochemical staining of thin paraffin‐embedded tissue sections from adult and aged nonloaded (NL) and loaded (L) tibias. (A) Prrx1 + cell number normalized to periosteal perimeter and (B) as the percentage of total cells. (C) PCNA + proliferating cell number normalized to periosteal perimeter and (D) the percentage of total cells. Representative images of the periosteum stained for Prrx1 (E–H) and PCNA (I–L) for all experimental groups. Black arrows denote positively stained cells. Scale bars = 50 µm.