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HA and PPA stimulate 35S‐GTPγS binding to membranes from mouse osteoblastic calvarial cells transfected with GPR109A but not GPR109B. Ligand‐induced 35S‐GTPγS binding was studied using membranes prepared from mouse fetal calvarial cells expressing mouse GPR109A (A) or GPR109B (B). Binding of 35S‐GTPγS was determined in the presence of increased concentration of nicotinic acid, acifran, HA, or PPA. HA = hippuric acid; PPA = 3‐(3‐hydroxyphenyl)‐propionic acid.
