Table 1.
Iron and Bone Status in 11, 29, and 53 Weeks of Age Hfe−/− Male Mice
| Age | 11‐weeks‐old | 29‐weeks‐old | 53‐weeks‐old | |||
|---|---|---|---|---|---|---|
| Strain | Hfe +/+ | Hfe −/− | Hfe +/+ | Hfe −/− | Hfe +/+ | Hfe −/− |
| n (males) | 6 | 4 | 6 | 7 | 5 | 7 |
| µCT Femur | ||||||
| BV/TV (%) | 13.9 ± 3.4 | 16.2 ± 3.3 | 15.1 ± 4.3 | 15.4 ± 3.7 | 6.6 ± 3.0 | 6.3 ± 2.4 |
| Tb.Th (µm) | 62.8 ± 3.3 | 61.3 ± 2.1 | 74.6 ± 4.9 | 74.0 ± 7.8 | 72.1 ± 6.9 | 70.1 ± 7.0 |
| Tb.Sp (µm) | 249.2 ± 13.8 | 220.5 ± 31.1 | 249.1 ± 28.0 | 247.0 ± 28.9 | 343.4 ± 69.2 | 311.0 ± 47.9 |
| Tb.N (mm‐1) | 2.2 ± 0.4 | 2.7 ± 0.6 | 2.0 ± 0.5 | 2.1 ± 0.5 | 0.9 ± 0.4 | 0.9 ± 0.4 |
| Cs.Th (µm) | 211.8 ± 11.3 | 211.7 ± 6.6 | 216.9 ± 3.8 | 207.0 ± 4.9 ** | 188.6 ± 14.9 | 188.5 ± 6.4 |
| µCT Vertebra | ||||||
| BV/TV (%) | 27.2 ± 2.2 | 23.5 ± 2.2 * | 32.9 ± 4.3 | 34.8 ± 5.8 | 20.2 ± 6.3 | 23.0 ± 4.7 |
| Tb.Th (µm) | 65.2 ± 5.8 | 63.2 ± 1.8 | 75.4 ± 4.1 | 77.7 ± 7.5 | 64.3 ± 4.8 | 65.2 ± 2.7 |
| Tb.Sp (µm) | 185.3 ± 4.2 | 174.2 ± 12.0 | 288.7 ± 32.7 | 277.9 ± 28.5 | 228.8 ± 38.0 | 205.7 ± 32.8 |
| Tb.N (mm‐1) | 4.0 ± 0.2 | 4.3 ± 0.1 | 4.4 ± 0.4 | 4.5 ± 0.5 | 3.1 ± 0.8 | 3.5 ± 0.7 |
| Serum bone parameters | ||||||
| P1NP (ng/mL) | 131.14 ± 41.96 | 107.19 ± 13.87 | 38.06 ± 10.6 | 39.95 ± 11.57 | 31.71 ± 5.71 | 35.19 ± 3.31 |
| CTX‐I (ng/mL) | 20.97 ± 2.66 | 13.18 ± 1.93 ** | 12.34 ± 2.3 | 12.86 ± 2.4 | 11.53 ± 2.74 | 12.76 ± 1.5 |
| Testosterone (ng/mL) | 4.49 ± 5.52 | 1.96 ± 2.58 | 7.59 ± 5.81 | 6.12 ± 6.3 | 3.52 ± 3.68 | 2.75 ± 2.88 |
| Iron levels | ||||||
| Plasma (µg/dL) | 133.6 ± 15.9 | 163.6 ± 14.6 * | 92.8 ± 14.1 | 151.3 ± 17.3 *** | 101.25 ± 19.0 | 149.3 ± 27.8 * |
| Liver (µg/g) | 178.5 ± 16.7 | 502.5 ± 84.6 ** | 219.8 ± 77.5 | 615.7 ± 130.8 *** | 256.6 ± 131.3 | 679.9 ± 172.6 * |
| Spleen (µg/g) | 1183.5 ± 296.8 | 1054.1 ± 344.9 | 907.8 ± 298.8 | 915.6 ± 273.7 | 1257.5 ± 672.1 | 823.7 ± 559.0 |
| Duodenum (µg/g) | 346.2 ± 114.8 | 212.1 ± 59.2 | 256.2 ± 74.2 | 220.8 ± 72.1 | 266.7 ± 44.8 | 265.5 ± 36.8 |
| Pancreas (µg/g) | 103.72 ± 60.8 | 74.4 ± 28.9 | n.d | n.d | n.d | n.d |
| Hematological indices | ||||||
| RBC (106/ mm3) | 8.05 ± 0.21 | 8.63 ± 0.11 *** | 8.88 ± 0.55 | 9.74 ± 0.56 * | 8.53 ± 0.34 | 8.5 ± 0.84 |
| Hgb (g/dL) | 15.35 ± 0.54 | 17.25 ± 0.33 *** | 14.93 ± 0.6 | 17.11 ± 0.87 *** | 14.4 ± 1.35 | 14.89 ± 2.02 |
| HCT (%) | 42.8 ± 1.4 | 48.62 ± 1.62 ** | 46.68 ± 2.04 | 53.87 ± 3.12 *** | 44.25 ± 1.57 | 47.22 ± 4.65 |
| MCV (µm 3) | 53.17 ± 0.75 | 56.5 ± 1.29 * | 53 ± 1.21 | 55 ± 0.95 ** | 53.0 ± 0.63 | 55.45 ± 1.81 *** |
| MCH (pg) | 19.08 ± 0.35 | 19.98 ± 0.21 ** | 16.85 ± 0.47 | 17.57 ± 0.35 * | 16.85 ± 1.15 | 17.56 ± 2.3 |
| MCHC (g/dL) | 35.85 ± 0.36 | 35.48 ± 0.5 | 32 ± 0.36 | 31.81 ± 0.34 | 32.47 ± 2.41 | 31.65 ± 3.86 |
Data were analyzed using GraphPad Prism software and results are shown as mean ± SD. For the statistical analysis, a nonparametric distribution and the Mann‐Whitney U test were used.
BV/TV = bone volume/tissue volume; Tb.Th = trabecular thickness; Tb.Sp = trabecular separation; Tb.N = trabecular number; Cs.Th = cortical thickness; P1NP = procollagen type 1 amino‐terminal propeptide; CTX‐I = C‐terminal telopeptide I; RBC = red blood cells; Hgb = hemoglobin; HCT = hematocrit; MCV = mean corpuscular volume; MCH = mean corpuscular hemoglobin; MCHC = mean corpuscular hemoglobin concentration; n.d. = no data available.
*
p values <.05
**
p values <.01
***
p values <.005.