Table 2.
Iron and Bone Status in 11‐, 29‐, and 53‐Week‐Old Hfe−/− Female Mice
| Age | 11 weeks old | 29 weeks old | 53 weeks old | |||
|---|---|---|---|---|---|---|
| Strain | Hfe +/+ | Hfe −/− | Hfe +/+ | Hfe −/− | Hfe +/+ | Hfe −/− |
| n (females) | 7 | 4 | 7 | 7 | 8 | 9 |
| µCT Femur | ||||||
| BV/TV (%) | 5.2 ± 2.6 | 5.8 ± 2.0 | 2.5 ± 0.8 | 3.6 ± 1.5 | 1.0 ± 0.6 | 1.3 ± 0.6 |
| Tb.Th (µm) | 54.7 ± 2.4 | 59.2 ± 4.2 | 58.3 ± 8.7 | 58.0 ± 9.2 | 62.1 ± 15.7 | 65.7 ± 11.9 |
| Tb.Sp (µm) | 315.2 ± 19.7 | 340.0 ± 26.1 | 384.8 ± 23.4 | 343.8 ± 32.6 * | 586.1 ± 102.2 | 522.0 ± 58.9 |
| Tb.N (mm‐1) | 0.9 ± 0.5 | 1.0 ± 0.3 | 0.4 ± 0.1 | 0.6 ± 0.2 | 0.2 ± 0.1 | 0.2 ± 0.1 |
| Cs.Th (µm) | 186.8 ± 7.8 | 187.2 ± 7.1 | 221.5 ± 8.7 | 220.0 ± 11.8 | 200.1 ± 13.7 | 204.5 ± 9.0 |
| µCT Vertebra | ||||||
| BV/TV (%) | 14.9 ± 2.3 | 15.1 ± 0.6 | 22.5 ± 3.6 | 25.2 ± 2.0 | 19.7 ± 4.4 | 22.4 ± 3.4 |
| Tb.Th (µm) | 49.1 ± 2.7 | 50.5 ± 1.5 | 73.3 ± 3.2 | 77.7 ± 4.3 | 74.3 ± 4.4 | 74.1 ± 3.8 |
| Tb.Sp (µm) | 224.7 ± 17.5 | 234.0 ± 14.4 | 288.7 ± 32.7 | 277.9 ± 28.5 | 331.2 ± 62.7 | 334.9 ± 56.2 |
| Tb.N (mm‐1) | 3.0 ± 0.4 | 3.0 ± 0.1 | 3.1 ± 0.5 | 3.3 ± 0.3 | 2.6 ± 0.5 | 3.0 ± 0.5 |
| Serum Bone Parameters | ||||||
| P1NP (ng/mL) | 130.3 ± 40.6 | 78.1 ± 12.4 ** | 29.4 ± 8.3 | 33.2 ± 4.3 | 33.9 ± 5.6 | 38.5 ± 7.2 |
| CTX‐I (ng/mL) | 21.7 ± 4.1 | 22.3 ± 6.7 | 12.2 ± 2.3 | 12.7 ± 2.8 | 12.0 ± 3.9 | 14.0 ± 2.1 |
| Iron levels | ||||||
| Plasma (µg/dL) | 140.7 ± 26.5 | 182.8 ± 24.8 * | 105.6 ± 26.3 | 166.2 ± 39.0 * | 118.7 ± 31.7 | 166.4 ± 24.4 * |
| Liver (µg/g) | 214.8 ± 45.2 | 942.7 ± 130.9 ** | 345.2 ± 82.2 | 999.9 ± 220.5 ** | 555.7 ± 269.3 | 1041.6 ± 127.8 ** |
| Spleen (µg/g) | 1800.0 ± 443.3 | 836.8 ± 177.6 ** | 1123.2 ± 255.7 | 875.8 ± 349.2 (0.07) | 1262.57 ± 401.57 | 847.0 ± 346.05 * |
| Duodenum (µg/g) | 284.0 ± 133.7 | 201.6 ± 99.9 | 272.5 ± 46.5 | 240.6 ± 58.7 | 317.8 ± 87.7 | 324.5 ± 112.8 |
| Pancreas (µg/g) | 103.7 ± 60.8 | 74.4 ± 28.9 | n.d. | n.d. | 123.7 ± 30.3 | 150.2 ± 34.5 |
| Hematological indices | ||||||
| RBC (106/ mm3) | 7.8 ± 0.3 | 8.7 ± 0.4 ** | 8.9 ± 0.9 | 9.5 ± 0.4 * | 8.5 ± 0.4 | 7.7 ± 0.5 |
| Hgb (g/dL) | 15.5 ± 0.5 | 17.2 ± 0.6 * | 15.0 ± 1.1 | 17.0 ± 0.6 ** | 15.7 ± 0.9 | 15.9 ± 0.8 |
| HCT (%) | 41.9 ± 1.0 | 48.5 ± 1.9 ** | 47.6 ± 1.8 | 52.5 ± 1.7 ** | 44.8 ± 2.6 | 42.9 ± 2.5 |
| MCV (µm3) | 53.9 ± 1.5 | 55.5 ± 0.6 | 52.0 ± 1.2 | 55.0 ± 1.0 ** | 53.0 ± 2.3 | 55.6 ± 2.1 |
| MCH (pg) | 19.9 ± 0.6 | 19.7 ± 0.3 | 16.7 ± 0.5 | 17.8 ± 0.4 ** | 18.6 ± 0.9 | 20.7 ± 1.0 * |
| MCHC (g/dL) | 37.0 ± 0.5 | 35.4 ± 0.3 ** | 32.4 ± 0.4 | 32.3 ± 0.5 | 35.1 ± 0.7 | 37.2 ± 2.4 |
Data were analyzed using GraphPad Prism software and results are shown as mean ± SD. For the statistical analysis, a nonparametric distribution and the Mann‐Whitney U test were used.
BV/TV = bone volume/tissue volume; Tb.Th = trabecular thickness; Tb.Sp = trabecular separation; Tb.N = trabecular number; Cs.Th = cortical thickness; P1NP = procollagen type 1 amino‐terminal propeptide; CTX‐I = C‐terminal telopeptide I; RBC = red blood cells; Hgb = hemoglobin; HCT = hematocrit; MCV = mean corpuscular volume; MCH = mean corpuscular hemoglobin; MCHC = mean corpuscular hemoglobin concentration; n.d. = no data available.
*
p values <.05.
**
p values <.01.