Table 1. Primers for RT-qPCR.
Chickpea identifiers, annotation NCBI, primer sequences, amplicon size and chromosome position. Primer PCR efficiency and PCR product Tm data represent mean values ± sd. PCR efficiencies (E) calculated according to the equation (1 + E) = 10slope.
| Chickpea ID NCBI | Annotation | Primer Sequence (5‘-3’) | PCR product size (bp) | Chr position | PCR E | PCR product Tm (°C) |
|---|---|---|---|---|---|---|
| LOC101503802 | kinesin-like protein FRA1 | F-TGCTTCCATTCCACCGAAGCCTG | 82 | Ca2: 23082299–23082381 | 2.02 ± 0.09 | 75.77 ± 0.25 |
| R-TTGCTTTTGCATGGCCCGTGGT | ||||||
| LOC101505941 | serine/threonine-protein kinase CTR1 | F-CGCCTGAGTGGATGGCTCCAGA | 98 | Ca2: 23219419–23219517 | 1.99 ± 0.07 | 78.06 ± 0.16 |
| R-GGTCACGAGTTCCCACAGGATCA | ||||||
| LOC101506693 | GATA transcription factor 25-like | F-AGGATTCTGGGCAGGACGACAG | 85 | Ca2: 23277155–23277240 | 1.94 ± 0.05 | 78 ± 0 |
| R-CGCCGCATCATTGGGGTCGATT | ||||||
| LOC101507659 | E3 ubiquitin-protein ligase UPL1-like | F-AGTGCTGCATCGCCAGTTATCCA | 73 | Ca2: 23441535–23441608 | 1.95 ± 0.08 | 77.88 ± 0.29 |
| R-GCCGAGCCTTGTCCTCCTTGCT | ||||||
| LOC101508507 | probable rhamnogalacturonate lyase C | F-CCAACGGGGTAGCGTTTGTGGT | 108 | Ca2: 23463108–23463216 | 1.96 ± 0.07 | 77 ± 0.11 |
| R-GCATCTCCTGGTGGTGCCAATCC | ||||||
| LOC101509037 | XIAP-associated factor 1-like | F-GGTGTCCGACGACGAAGG | 80 | Ca2: 23476846–23476926 | 1.98 ± 0.09 | 77.5 ± 0 |
| R-ACAGCAATACCGGTGATGGC | ||||||
| LOC101509359 | MADS-box transcription factor 23-like | F-ACGGTTTGTTGAAGAAGGCGAAGG | 116 | Ca2: 23551043–23551159 | 1.95 ± 0.06 | 77.43 ± 0.17 |
| R-TGATCTCATGCTGGTGCTAGCGAA | ||||||
| LOC101510206 | serine hydroxymethyltransferase, mitochondrial-like | F-GGCTCGAGGGTTGAGAAGGTGT | 94 | Ca2: 23674613–23674707 | 1.99 ± 0.07 | 77.16 ± 0.23 |
| R-TGCCCCCAGGAACCATAGCAGA | ||||||
| LOC101510544 | 26S protease regulatory subunit 8 homolog A-like | F-GCCAGGGAGCATGCACCATCAA | 105 | Ca2: 23699688–23699793 | 1.97 ± 0.07 | 78.52 ± 0.10 |
| R-GCGCTGCACCTCACTATCACCA | ||||||
| LOC101511605 | CBL-interacting serine/threonine-protein kinase 8 | F-TGCTTCGGACAACTTGCGGGAC | 98 | Ca2: 23829711–23829809 | 1.97 ± 0.05 | 76.74 ± 0.23 |
| R-CCCACACGACCAAACATCCGCT | ||||||
| LOC101495287 | auxin-responsive protein IAA8-like | F-TGAGAGGCCTCCTGGTGTCTGTG | 92 | Ca2: 23950935–23951027 | 1.97 ± 0.07 | 76.71 ± 0.25 |
| R-CCTGTGCCTTGGTAGCTGGTGC | ||||||
| LOC101495941 | MATE efflux family protein 5-like | F-GGTGGGGTGGCAATAGCAATGGT | 109 | Ca2: 24038256–24038365 | 1.94 ± 0.06 | 74.09 ± 0.21 |
| R-AGCTTGGTGGGACCCATGATTGT | ||||||
| LOC101496824 | sucrose transport protein SUC4 | F-ATCTGGCTTTGCGGCCCAGTTT | 88 | Ca2: 24044382–24044470 | 1.93 ± 0.06 | 79.64 ± 0.14 |
| R-GTCGACCGAATCGGCTTTGGCA | ||||||
| LOC101497351 | bidirectional sugar transporter N3-like | F-ATGCATGGTTCCCTTCGAGTCCA | 89 | Ca2: 24085883–24085972 | 1.96 ± 0.08 | 76.84 ± 0.25 |
| R-GCCACAATGCATAGAGGTGCTGC | ||||||
| LOC101497678 | glycerol-3-phosphate dehydrogenase [NAD(+)] 2, chloroplastic | F-AACCGGTCTGTCAGGAACTGGAG | 93 | Ca2: 24092611–24092704 | 1.96 ± 0.07 | 76.7 ± 0 |
| R-TCGCCTGATCCAAGACGCACAC | ||||||
| LOC101502928 | WRKY transcription factor 55 | F-AGCACCATTATCATATCCACCAC | 80 | Ca2: 24128013–24128093 | 1.96 ± 0.06 | 68.87 ± 0.10 |
| R-ACAATTGGGGAGAAATGGTGGT | ||||||
| LOC101499218 | E3 ubiquitin-protein ligase SHPRH | F-CAACACGTGGTCCTTGTTGAGCC | 80 | Ca2: 24209104–24209184 | 1.91 ± 0.07 | 77.7 ± 0.14 |
| R-TGTCCAATCCGATGTACGCGACT | ||||||
| LOC101501552 | probable inactive receptor kinase At5g58300 | F-GGTGGGTGAAGTCTGTGGTTTCTGA | 108 | Ca2: 24365119–24365227 | 1.95 ± 0.10 | 76.29 ± 0.16 |
| R-TCTGAAGCATCTGCACCATCTCCTC | ||||||
| LOC101510320 | pathogenesis-related protein 5 | F-GCTGCACATTTGATGCAACGGGA | 89 | Ca2: 24740222–24740311 | 1.93 ± 0.05 | 76.88 ± 0.24 |
| R-TGGAGCTGCACCATTCCCATCAC | ||||||
| LOC101499873 | TMV resistance protein N-like | F-TGACCTTACGTGGCACCGCTAT | 126 | Scaffold | 1.97 ± 0.06 | 76.74 ± 0.22 |
| R-GCTGCGGATCATTTGACAACCCA | ||||||
| LOC101490851 | 20 kDa chaperonin, chloroplastic-like | F-CCAGGGTCTGTGGATGAGGAAGG | 99 | Ca7: 3352808 - 3352906 |
1.96 ± 0.06 | 74.91 ± 0 |
| R-GCCCTTGAAGTCATTCCCCGCA | ||||||
| LOC105852647 | MLP-like protein 28 | F-TGGTGGAGATATTGATGAGCACT | 80 | Ca7: 45480380 - 45480459 |
1.97 ± 0.08 | 72.19 ± 0.25 |
| R-CGGAAGCACTACCATCAGCC |