Fig 3. Differential recruitment of immune cells to the alveolar airspace in neonatal animals.

Mouse pups received 1 x10⁶ Colony Forming Units of LGG intranasally on Days 1 and 2 of life. On Day 3, pups were infected intranasally with PR-8 influenza. (A) Histopathology was done at 3 and 6 days post-infection, which demonstrated similar bronchopulmonary infiltrates in both sham and LGG treated neonates. H&E Stain, representative figures. Arrows indicate areas of bronchopulmonary infiltration. Clinical severity scoring was performed (B). In separate experiments, cell counts for the specified cell types from (C) whole lung tissue and (D) bronchoalveolar lavage was determined by flow cytometry. Data from 3 independent experiments.