Fig 1. Position of different primers and spacer sequence in the chilli leaf curl virus (ChiLCV) genome and plan for in vitro cleavage assay.

a) Three genomic regions (green color) of the ChiLCV were selected to identify the spacer, including i. Intergenic region (IR) with the origin of replication, ii. Overlapping regions between V2 and V1 genes (V2/V1) and iii. Overlapping regions between C1 and C4 genes (C1/C4); b) schematic representation of in vitro cleavage assay.