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. 2019 Oct 23;14(10):e0223765. doi: 10.1371/journal.pone.0223765

Fig 2. In vitro cleavage assay.

Fig 2

a) Amplification of the target region from the ChiLCV (lane 1–3 indicates three replicates of same sample) b) In-vitro cleavage assay using individual gRNA-Cas9 ribonucleoprotein (RNP) complex. The 1.5-kb viral genomic fragment containing all six target spacer sequences was treated by six individual gRNA-Cas9 RNP complexes. c) In-vitro cleavage assay using multiplexed gRNA-Cas9 RNP complex. The 1.5-kb viral genomic fragment containing all six target spacer sequences was treated by duplex or triplex gRNA-Cas9 RNP complexes. Uncut: 1.5-kb viral genomic fragment without the RNP treatment.