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. 2019 Oct 23;14(10):e0223765. doi: 10.1371/journal.pone.0223765

Fig 3. In planta expression assay.

Fig 3

a) Inoculation positions of gRNA-Cas9 construct and ChiLCV, b) qPCR assay to understand reduction of viral replication, c) qRT-PCR assay for gRNA expression analysis, d) qRT-PCR assay for Cas9 expression analysis. For qPCR and qRT-PCR, three replicates were used for each data point. The error bar denotes SEM. Stars indicate significant difference (student’s unpaired t-test) of ChiLCV expression levels between gRNA-Cas9 treated and non-treated plants (*P< 0.05, **P< 0.01, ***P< 0.001).