Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Oct 23;14(10):e0223765. doi: 10.1371/journal.pone.0223765

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.

PMC Copyright notice

Fig 5 — a) T7EI assay from gRNA treated ChiLCV challenged plant along with ChiLCV inoculated plant. Lane M, GeneRuler 1kb plus DNA ladder. Samples in lane 1 through 4 were all amplified using primers ChiLCV-C1/V1q F &ChiLCV-IRR and then treated with T7E1. Lane 1, amplicon from mock vector treated ChiLCV inoculated N. benthamiana. Lane 2, amplicon from gRNA5+4-Cas9 construct treated ChiLCV challenged plants. Lane 3, amplicon from gRNA5+2-Cas9 construct treated ChiLCV challenged plants. Lane 4, amplicon from gRNA1+2-Cas9 construct treated ChiLCV challenged plants. b) Control heteroduplex DNA (C) from the kit showed cleavage of the control heteroduplex by the T7EI.