Fig 5. Antigen presentation and activation of bone marrow-derived dendritic cells after infection with viRNA-mOVA.
Mouse bone marrow-derived dendritic cells were infected with sucrose purified viRNA-mOVA WR, sucrose purified mOVA complexed with cationic liposomes as a control, virus alone or non-treated cells as a mock. A) Antigen presentation was determined by flow cytometry using APC-conjugated anti-H-2Kb bound to SIINFEKL. B) Dendritic cell activation and C) migration marker expression was determined by flow cytometry using antibodies against murine CD86 and murine CD103, respectively. Each bar is the mean ± SEM of technical triplicates.