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. 2019 Oct 3;34(10):1999–2008. doi: 10.1093/humrep/dez139

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© The Author(s) 2019. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Contribution of ECM proteins to decidual stiffness. Analysis of single-cell RNA sequencing of first trimester decidua (data from Vento-Tormo et al., 2018) show normalized expression levels of RNA transcripts encoding selected ECM components. Panel (A) shows transcripts where scaled Log-transformed, normalized expression levels, LogN_e in EVT 1 compared with three stromal cell subsets: DS1, DS2, and DS3 identified by scRNAseq. Data from Vento-Tormo et al., 2018. Panel (B) shows ECM transcripts where expression in EVT is more that in decidual subsets, LogN_e1. Decidual stromal cell subsets DS1, DS2, and DS3 primarily express fibrillar collagens that provide mechanical strength. EVT cells primarily express ECM proteins that make up basement membrane. Fibrillin 1, a glycoprotein that is known to provide structural support in connective tissues, was found to be expressed by both EVT and stromal cells. (C) Protein expression of fibrillin 1 was confirmed by IHC on EVT cells. Serial sections of decidua basalis were stained for cytokeratin 7 (uterine glands and EVT) and fibrillin 1 (EVT and decidual stromal cells). Fibrillin panel shows staining of matching area to box on cytokeratin panel. White arrows indicate EVT with co-expression of both cytokeratin 7 and fibrillin 1 in serial sections (N = 3 biological replicates). Scale bars are 100 μm (cytokeratin panel) and 50μm (fibrillin panel).

Inline graphic — Contribution of ECM proteins to decidual stiffness. Analysis of single-cell RNA sequencing of first trimester decidua (data from Vento-Tormo et al., 2018) show normalized expression levels of RNA transcripts encoding selected ECM components. Panel (A) shows transcripts where scaled Log-transformed, normalized expression levels, LogN_e in EVT 1 compared with three stromal cell subsets: DS1, DS2, and DS3 identified by scRNAseq. Data from Vento-Tormo et al., 2018. Panel (B) shows ECM transcripts where expression in EVT is more that in decidual subsets, LogN_e1. Decidual stromal cell subsets DS1, DS2, and DS3 primarily express fibrillar collagens that provide mechanical strength. EVT cells primarily express ECM proteins that make up basement membrane. Fibrillin 1, a glycoprotein that is known to provide structural support in connective tissues, was found to be expressed by both EVT and stromal cells. (C) Protein expression of fibrillin 1 was confirmed by IHC on EVT cells. Serial sections of decidua basalis were stained for cytokeratin 7 (uterine glands and EVT) and fibrillin 1 (EVT and decidual stromal cells). Fibrillin panel shows staining of matching area to box on cytokeratin panel. White arrows indicate EVT with co-expression of both cytokeratin 7 and fibrillin 1 in serial sections (N = 3 biological replicates). Scale bars are 100 μm (cytokeratin panel) and 50μm (fibrillin panel).