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. 2019 Oct 3;34(10):1999–2008. doi: 10.1093/humrep/dez139

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© The Author(s) 2019. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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An order of magnitude difference in apparent elastic modulus between Matrigel® and decidua basalis. AFM was used to determine stiffness of five batches of 100% Matrigel® (undiluted) and at dilutions to 80%, 60%, and 40% with EVT medium. Matrigel® is an artificial ECM basement membrane and the most common substrate used in 3D migration assays with EVT and culture of organoids. Nonlinear regression analysis was used to fit the data and determine the equation of the line. Matrigel® stiffness was similar to that for endometrium and first trimester placenta but much lower than that for decidua basalis.