Skip to main content
. 2019 Oct 15;8:e49309. doi: 10.7554/eLife.49309

Figure 3. Elevated IIS in late germarium induces Myc protein to prime mtDNA replication and mitochondrial respiration.

(A) Representative germaria from wt or chico1/KG mutant flies incorporating EdU and stained with anti-1B1. Arrowheads indicate EdU incorporation into the mtDNA of germ cells. Scale bars, 10 μm. (B) Quantification of the mitochondrial EdU puncta areas relative to the areas of germline cysts from wt or chico1/KG mutant flies. n = 11 cysts each column (left to right). Error bars represent SEM. *p<0.005. (C) Quantifications of COX activity in region 2B cysts from wt or chico1/KG mutant flies. The activities are normalized to that of region 2B cysts from wt. n = 10 germaria for each genotype. Error bars represent SEM. *p<0.005. (D) Germaria from wt ovaries stained with anti-AKT and anti-p-AKT (S505). p-AKT staining is low in both GSCs and dividing cysts, while increased from region 2B germ cells. In contrast, AKT staining is uniform in the germarium. Scale bars, 10 μm. (E) Germaria from wt or chico1/KG mutant ovaries endogenously expressing Myc-GFP stained with anti-GFP, anti-1B1, and DAPI. Scale bars, 10 μm. (F) Quantification of relative Myc-GFP intensity in germarium region 2B from wt or chico1/KG mutant ovaries. n = 10 germaria for each genotype. Error bars represent SEM. *p<0.005.

Figure 3—source data 1. Relative mtDNA area, COX activity and Myc-GFP intensity in the germarium.
elife-49309-fig3-data1.xlsx (12KB, xlsx)
DOI: 10.7554/eLife.49309.011

Figure 3.

Figure 3—figure supplement 1. IIS activity in the ovary.

Figure 3—figure supplement 1.

(A) A low-magnification image of ovarioles stained with anti-p-AKT and anti-1B1. Note that IIS activity is low in early germarium stages, becomes high from late germarium, and decreases from the stage-10 egg chamber, the same pattern as Myc protein. Scale bar, 100 μm.