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. 2019 Nov;25(11):2131–2133. doi: 10.3201/eid2511.190431

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Light microscopic examination of human embryonic lung cells inoculated for 28 days from a clinical sample from a 47-year-old man with Pott’s disease and systemic tuberculosis, France. A) Cytopathic effect consisting of cell lysis caused by growing Mycobaterium tuberculosis. Original magnification ×200. B) M. tuberculosis mycobacteria observed after Ziehl-Neelsen staining. Original magnification ×1,000, by oil immersion. C) Absence of any cytopathic effect in negative control cell culture. Original magnification ×200. D) Absence of any mycobacteria in the negative control cell culture after Ziehl-Neelsen staining. Original magnification ×1,000 by oil immersion.