Table 1.
Morphological events occurring during replication of T. gondii parasite tachyzoites.
| Time count (hours:minutes) |
Events | Images |
|---|---|---|
| 0 to 1:15 | G1 phase (may vary in length up to 4 hours)* | |
| Centriole, Golgi and apicoplast closely associated at the apical end of the nucleus† | Fig. 2A–C | |
| 1:15 to 1:45 | First morphological changes | |
| Centriole migrates away from the apical end‡ | Fig. 2F and Fig. 7 (magenta, increasing) | |
| Golgi elongates‡ | Fig. 2D and Fig. 7 (yellow, increasing) | |
| Apicoplast migrates to juxtanuclear region, associates with mitochondrion, and elongates§ | Fig. 2E–F, Fig. 5B–C and Fig. 7 (green, increasing) | |
| 1:45 to 3:0 | Initial stages of mitotic division | |
| Centriole divides‡ | Fig. 2I and Fig. 7 (magenta, peak) | |
| Golgi divides‡ | Fig. 2G and Fig. 7 (yellow, peak) | |
| Initiation of DNA replication¶ | Fig. 7 (turquoise, increasing) | |
| Apicoplast continues to elongate§ | Fig. 2H–I and Fig. 7 (green, increasing) | |
| Centriole returns to the apical end and associates with apicoplast‡ | Fig. 2L and Fig. 7 (magenta, decreasing) | |
| 3:0 to 3:30 | Establishment of the daughter cytoskeleton | |
| Daughter conoids form** | Fig. 7 (purple, increasing) | |
| Spindle poles and intranuclear spindle form ** | Fig. 7 (purple, increasing) | |
| 1.8N DNA content¶ | Fig. 7 (turquoise) | |
| Inner membrane complex assembly begins†† | Fig. 2J–K, Figs 3, 4, Fig. 5A and Fig. 7 (purple) | |
| Apical ruffling of endoplasmic reticulum‡‡ | Figs 3 and 7 (brown, increasing) | |
| 3:30 to 4:30 | Organellar partitioning (early stages) | |
| Daughter inner membrane complexes elongate†† | Fig. 2M–N, Figs 3, 4, Fig. 5A and Fig. 7 (purple) | |
| Apicoplast partitioned and divides§ | Fig. 2N–O and Fig. 7 (green) | |
| Nucleus partitioned and divides§§ | Fig. 2N–O and Fig. 7 (blue) | |
| ER partitioned between daughters‡‡ | Figs 3 and 7 (brown) | |
| Initiation of de novo rhoptry and microneme biogenesis¶¶ | Figs 4 and 7 (orange, increasing) | |
| Mitochondrion forms branches | Fig. 5A and Fig. 7 (red, increasing) | |
| 4:30 to 6:30 | Organellar partitioning (late stages) | |
| Continued partitioning of ER‡‡ | Figs 3 and 7 (brown, decreasing) | |
| Continued rhoptry and microneme biogenesis¶¶ | Figs 4 and 7 (orange, increasing) | |
| Daughter inner membrane complexes complete†† | Figs 3, 4, Fig. 5A and Fig. 7 (purple) | |
| Mitochondrion enters daughter scaffolds | Fig. 5A and Fig. 7 (red, decreasing) | |
| 6:30 | Emergence of daughter parasites | |
| Daughter parasites bud from mother, picking up plasma membrane‡‡ | Fig. 3 and Fig. 5A | |
| Residual material left behind (including waste, ER, mitochondrion, plasma membrane etc.)‡‡ | Fig. 3, Fig. 5A and Fig. 6 |